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There are many methods provided by manufacturers to facilitate Incubator self-decontamination. Three of the most common methods are:
UV sterilization

Moist heat sterilization

Dry heat sterilization

This article will examine these three types of sterilization in detail.


1. UV Sterilization

DNA nucleotides harbor the kinds of conjugated bonds that absorb UV light. UV sterilization generates an antimicrobial effect by the damage it causes to a microorganism’s DNA when aromatic nucleotides absorb high energy photons. This can make UV sterilization an effective solution to reduce contamination in an incubator chamber.

However, there are significant drawbacks. Your light source would need to have unrestricted access to all surfaces of the Multifunctional Incubator chamber, shelves, and shelf mounting hardware. Shadowed regions will not be decontaminated by UV light. Also, a common method for microorganisms to survive UV exposure is through enhanced DNA repair mechanisms. In this case, survivors of a UV cycle will be more likely to survive repeat treatments.

Plus, UV light is generally not effective in destroying endospores. Microorganisms which survive the UV decontamination process will potentially have the opportunity to form monocultures and increase their likelihood to reach quorum. This is unless UV sterilization is combined with other methods of incubator decontamination, such as a tear-down and washing of all surfaces, a dispersed chemical treatment, or an effective high-heat cycle.

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